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The Porcine circovirus type 2 (PCV2) ELISA kit is used to detect circovirus type 2 specific antibodies in pig serum as a serological aid to assess the immunization status or infection status of PCV2 in pig farms.
The kit uses an indirect ELISA method in which purified PCV2 antigen is pre-encapsulated on enzyme-labelled strip microwells. In the test, diluted serum to be tested is added and after warming, if the sample contains PCV2 specific antibodies, they bind to the PCV2 antigen on the coated plate. After washing to remove unbound antibodies and other components, the enzyme-labelled secondary antibody is added to specifically bind to the antigen-antibody complex on the test plate, and then after washing to remove unbound enzyme markers, TMB substrate solution is added to the microtiter wells. The reaction was terminated by the addition of termination solution and the absorbance OD value of the reaction wells was measured at 450nm using an enzyme marker.